Optimized DNA sequence encoding mature chain of human Olfactomedin-4 (OLFM4) (Asp 21 - GLN510) including an C terminal His tag was expressed in HEK293 cells.
Recombinant human Olfactomedin-4 is a protein consisting of 495 amino acid residue subunits,due to glycosylation migrates as an approximately 65kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human Olfactomedin-4 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Olfactomedin-4 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
OLFM4 (also called hGC-1 and
GW112) is a member of the
olfactomedin family, which
is characterized by the
presence of an olfactomedin
domain in the C terminus
proteins have distinct
tissue expression patterns
and diverse cellular
OLFM4 is expressed in
epithelial cells of the
prostate, small intestine,
colon, and cells in the bone
marrow. It is also expressed
in several cancers and up-
regulated in epithelial
cells during inflammation
olfactomedin 4 inhibits cathepsin c-mediated protease activities, thereby modulating neutrophil killing of staphylococcus aureus and escherichia coli in mice
2460 - 2467.
olfactomedin 4 defines a subset of human neutrophils
J. Leukoc. Biol.,
495 - 500.
olfactomedin 4 suppresses prostate cancer cell growth and metastasis via negative interaction with cathepsin d and sdf-1
986 - 994.
olfactomedin 4 is a novel target gene of retinoic acids and 5-aza-2'-deoxycytidine involved in human myeloid leukemia cell growth, differentiation, and apoptosis
4938 - 4947.
olfactomedin 4, an indicator of the existence of neutrophil subsets.
Blood (ASH Annual Meeting Abstracts),