Optimized DNA sequence encoding extracellular domain of Human Neutrophil gelatinase-associated lipocalin-NGAL (GLN21-GLY198 ) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human NGAL is a protein consisting of 185 amino acid residue subunits,due to glycosylation migrates as an approximately 25kDa protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to bind Iron dihydroxybenzoic acid and was measured to be > 1.5uM .
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Lipocalin-2 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant NGAL can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
associated lipocalin (NGAL),
a 25 kDa amino acid protein,
is a member of the lipocalin
family largely expressed in
activated neutrophils and
tubular epithelial cells in
response to inflammation .
NGAL plays an important role
in innate immunity.
Accumulating evidence also
has established both urinary
and plasma NGAL levels as
predictive biomarkers for
acute kidney injury (AKI)
and its outcomes in varied
groups of patients including
pediatric and adult
populations, critically ill
patients and contrast-
induced nephropathy . NGAL
may originate from both
renal and extra-renal sites
interleukin-17-induced protein lipocalin 2 is dispensable for immunity to oral candidiasis
1030 - 1035.
muc4-mediated regulation of acute phase protein lipocalin 2 through her2/akt/nf-b signaling in pancreatic cancer
Clin. Cancer Res.,
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the induction of Lipocalin-2 expression in vivo and in vitro
J. Biol. Chem.,
the bacteriostatic protein lipocalin 2 is induced in the central nervous system of mice with west nile virus encephalitis
679 - 689.
lipocalin 2 induces the epithelial–mesenchymal transition in stressed endometrial epithelial cells: possible correlation with endometriosis development in a mouse model
179 - 187.