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Human IL-10 receptor beta (IL10RB) Recombinant

IL-10RB

accession:  Q08334
   Size A     50ug   $ 280
   Size B      200ug  $620
   Size C      1000ug  $ 2750
Domain  :  Extracellular
Gene  :  IL10RB
Catalog no. :  RKQ08334
Source:
DNA sequence encoding extracellular domain of human IL-10RB fused to a C-terminal polyHis tag was expressed in HEK cells.

 
Molecular weight:

Recombinant Human Interleukin-10RB  is a monomer consisting of 212 amino acid residue subunits, and migrates as an approximately 46 kDa protein under reducing conditions in SDS-PAGE.

 
Purity:
>90%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The activity was determined by its ability to bind Human IL-28B in functional ELISA .

 
Protein Sequence:
        10         20         30         40         50         60 
MAWSLGSWLG GCLLVSALGM VPPPENVRMN SVNFKNILQW ESPAFAKGNL TFTAQYLSYR 

        70         80         90        100        110        120 
IFQDKCMNTT LTECDFSSLS KYGDHTLRVR AEFADEHSDW VNITFCPVDD TIIGPPGMQV 

       130        140        150        160        170        180 
EVLADSLHMR FLAPKIENEY ETWTMKNVYN SWTYNVQYWK NGTDEKFQIT PQYDFEVLRN 

       190        200        210        220        230        240 
LEPWTTYCVQ VRGFLPDRNK AGEWSEPVCE QTTHDETVPS WMVAVILMAS VFMVCLALLG 

       250        260        270        280        290        300 
CFALLWCVYK KTKYAFSPRN SLPQHLKEFL GHPHHNTLLF FSFPLSDEND VFDKLSVIAE 

       310        320 
DSESGKQNPG DSCSLGTPPG QGPQS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Interleukin-10 receptor beta was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

IL-10 receptor

IL-10 is produced by mouse Th2-cells following their stimulation by lectins. The main source for B-cell derived IL10 in mice are Ly1 B-cells that express CD5 (Ly1) and CD11.In humans IL10 is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones after both antigen-specific and polyclonal activation, by B- cell lymphomas, and by monocytes following cell activation by bacterial lipopolysaccharides and mast cells.IL10 is a homodimeric protein with subunits having a length of 160 amino acids. Human Interleukin10 shows 73 % amino acid homology with mouse Interleukin-10. The murine IL-10 receptor has been cloned. This receptor is a protein of approximately 110 kDa that binds murine IL10 specifically. This receptor is structurally related to receptors for IFN. The CD nomenclature for this receptor is CDw210. IL10 inhibits the synthesis of a number of cytokines such as IFN-gamma, IL2 and TNF-beta in Th1 T-helper subpopulations of T-cells but not of Th2 T-helper cells. This activity is antagonized by IL4. The inhibitory effect on IFN- gamma production is indirect and appears to be the result of a suppression of IL12 synthesis by accessory cells. In the human system, IL10 is produced by, and down-regulates the function of Th1 and Th2 cells.

Related Publications:
genetic variation in the il-10 pathway modulates severity of acute graft-versus-host disease following hematopoietic cell transplantation: synergism between il-10 genotype of patient and IL-10 receptor genotype of donor
Blood, Dec 2005; 106: 3995 - 4001.
association of il-10 and IL-10 receptor gene polymorphisms and graft-versus-host disease following hematopoietic cell transplantation.
Blood (ASH Annual Meeting Abstracts), Nov 2004; 104: 421.
the expanded family of class ii cytokines that share the IL-10 receptor-2 (il-10r2) chain
J. Leukoc. Biol., Aug 2004; 76: 314 - 321.
cutting edge: il-26 signals through a novel receptor complex composed of il-20 receptor 1 and IL-10 receptor 2
J. Immunol., Feb 2004; 172: 2006 - 2010.
inhibition of IL-10 receptor function in alveolar macrophages by toll-like receptor agonists
J. Immunol., Feb 2004; 172: 2613 - 2620.




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