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Human Interferon alphacon-1 Recombinant

IFN-alpha

accession:  P01563
   Size A     10ug   $ 70
   Size B      50ug  $160
   Size C      1mg  $ 880
Domain  :  four helix cytokine core
Gene  :  IFNA2
Catalog no. :  RKP015632
Source:
Optimized DNA sequence encoding Human Interferon-alpha concensus mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Recombinant Interferon alpha concensus  is a disulfide-linked monomeric protein consisting of 166 amino acid residue subunits, and migrates as an approximately 19 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>97%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The activity was determined by a viral resistance assay  of Human WISH cells, and was found to be in the range of  1x108 IU/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MALTFALLVA LLVLSCKSSC SVGCDLPQTH SLGSRRTLML LAQMRKISLF SCLKDRHDFG 

        70         80         90        100        110        120 
FPQEEFGNQF QKAETIPVLH EMIQQIFNLF STKDSSAAWD ETLLDKFYTE LYQQLNDLEA 

       130        140        150        160        170        180 
CVIQGVGVTE TPLMKEDSIL AVRKYFQRIT LYLKEKKYSP CAWEVVRAEI MRSFSLSTNL 

       190 
QESLRSKE 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Interferon alpha 2a was lyophilized from a 0.2 μm filtered PBS solution pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

interferon alpha

All known subtypes of IFN- alpha show the same antiviral antiparasitic, antiproliferative activities. Human IFN-alpha is also a potent antiviral substance in murine, porcine, and bovine cell systems. IFN-alpha forms are produced by monocytes/macrophages, lymphoblastoid cells, fibroblasts, and a number of different cell types following induction by viruses, nucleic acids, glucocorticoid hormones, and low-molecular weight substances. All IFN-alpha subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino- terminal ends are variable. Many IFN-alpha subtypes differ in their sequences at only one or two positions. IFN-alpha and IFN-beta are thought to bind to the same receptor. Signal transduction mechanisms elicited after binding of IFN-alpha to its receptors involves tyrosine phosphorylation (see also: PTK; protein tyrosine kinase) of various non- receptor tyrosine kinases belonging to the Janus kinases.

Related Publications:
cytokines and angiogenic factors in patients with metastatic renal cell carcinoma treated with interferon-: association of pretreatment serum levels with survival
Ann. Onc., Oct 2009; 20: 1682 - 1687.
sequential multiple-assignment randomized trial design of neurobehavioral treatment for patients with metastatic malignant melanoma undergoing high-dose interferon-alpha therapy
Clinical Trials, Sep 2009; 10.1177/1740774509344633.
role of schlafen 2 (slfn2) in the generation of interferon -induced growth inhibitory responses
J. Biol. Chem., Sep 2009; 284: 25051 - 25064.
palmitoylation of interferon- (ifn-) receptor subunit ifnar1 is required for the activation of stat1 and stat2 by ifn-
J. Biol. Chem., Sep 2009; 284: 24328 - 24340.
lack of association between the interferon- signature and longitudinal changes in disease activity in systemic lupus erythematosus
Ann Rheum Dis, Sep 2009; 68: 1440 - 1446.




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product_icon_8.jpg Human Interferon alphacon-1 Recombinant
 
 
 
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