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Human Interleukin-24 Recombinant

IL-24

accession:  Q13007
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 3900
Domain  :  4 Helix Cytokine
Gene  :  IL24
Catalog no. :  RKQ13007
Source:
Optimized DNA sequence encoding Human Interleukin-24 mature chain including a C-terminal His tag was expressed in HEK293 cells.

 
Molecular weight:

Native human IL-24 has a calculated molecular mass of approximately 18.5 kDa. 

Recombinant IL-24 is a disulfide-linked monomeric protein consisting of 165 amino acid residue subunits, and migrates as an approximately 35kDa protein due to glycosylation under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Activity was determined by the ability to inhibit growth of human A549 cancer cells at concentrations ranging between 50-100 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MNFQQRLQSL WTLARPFCPP LLATASQMQM VVLPCLGFTL LLWSQVSGAQ GQEFHFGPCQ 

        70         80         90        100        110        120 
VKGVVPQKLW EAFWAVKDTM QAQDNITSAR LLQQEVLQNV SDAESCYLVH TLLEFYLKTV 

       130        140        150        160        170        180 
FKNYHNRTVE VRTLKSFSTL ANNFVLIVSQ LQPSQENEMF SIRDSAHRRF LLFRRAFKQL 

       190        200 
DVEAALTKAL GEVDILLTWM QKFYKL 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Interleukin-24 was lyophilized from  0.2μm filtered solution in PBS, pH 7.4 including 5% (w/v) trehalose.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

IL-24

IL-24 (melanoma differentiation-associated gene-7 (mda-7)), a member of the IL-10 cytokine family, possesses the properties of a classical cytokine as well as tumor suppressor effects. The exact role of IL-24 in the immune system has not been defined but studies have indicated a role for IL-24 in inflammatory conditions such as psoriasis. The tumor suppressor effects of IL-24 include inhibition of angiogenesis, sensitization to chemotherapy, and p38 mitogen-activated protein kinase (MAPK)-mediated apoptosis. Current knowledge on the regulation of IL-24 expression is sparse. The melanoma differentiation- associated gene-7 (mda-7) was discovered through subtraction hybridization of cDNA libraries prepared from melanoma cells. As mda-7 shares considerable structural and sequence homology with IL-10 cytokine family members it was recognized as a member of this family and renamed interleukin-24 (IL-24). IL-24 signals through a heterodimeric receptor complex consisting of the IL-20Rβ subunit together with either the IL-20Rα subunit or the IL-22Rα subunit. The IL-20Rα/IL-20Rβ complex is shared with IL-19 and IL-20 whereas the IL- 22Rα/IL-20Rβ complex is shared with only IL-20. Binding of IL-24 as well as IL-19 and IL-20 to the receptor complexes results in signal transducer and activator of transcription 3 (STAT3) -activation.

Related Publications:
a serotype 5/3 adenovirus expressing mda-7/IL-24 infects renal carcinoma cells and promotes toxicity of agents that increase ros and ceramide levels
Mol. Pharmacol., Nov 2010; 10.1124/mol.110.069484.
a single intramuscular injection of aav-8 vector expressing mda7/IL-24 efficiently suppresses tumor growth mediated by multiple anti-cancer mechanisms in lymphoma model mice.
Blood (ASH Annual Meeting Abstracts), Nov 2010; 116: 1474.
mechanism by which mcl-1 regulates cancer-specific apoptosis triggered by mda-7/IL-24, an il-10–related cytokine
Cancer Res., Jun 2010; 70: 5034 - 5045.
IL-24 transgenic mice: in vivo evidence of overlapping functions for il-20, il-22, and IL-24 in the epidermis
J. Immunol., Feb 2010; 184: 1793 - 1798.
perk–dependent regulation of ceramide synthase 6 and thioredoxin play a key role in mda-7/IL-24–induced killing of primary human glioblastoma multiforme cells
Cancer Res., Feb 2010; 70: 1120 - 1129.




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