Optimized DNA sequence encoding Human extracellular domain of human CD112 including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant CD112 is a monomer protein consisting of 335 amino acid residue subunits, due to glycosylation migrates as an approximately 48 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity of Recombinant human CD112 was measured in a functional ELISA assay, immobilized recombinant human CD112 at 20 μg/ml ( 100μl/well ) can bind biotinylated DNAM1 with a linear range of 0.08 - 3 μg/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD112 is supplied as a 0.2 μm filtered PBS solution, pH7.2.
Recombinant CD112, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Nectins are a small family
cell adhesion molecules
(CAMs) that control cell
adhesion, proliferation, and
is a 60 or 65 kDa type I TM
glycoprotein that is found
on a variety of cell types.
is known to bind the
virus, and herpes simplex
It does not bind poliovirus.
As a cell
adhesion molecule, Nectin2
will form cishomodimers
, followed by transdimers
mast cell costimulation by cd226/CD112 (dnam-1/nectin-2): a novel interface in the allergic process
J. Biol. Chem.,
27190 - 27196.
expression of the dnam-1 ligands, nectin-2 (CD112) and poliovirus receptor (cd155), on dendritic cells: relevance for natural killer-dendritic cell interaction
2030 - 2036.
analysis of the receptor-ligand interactions in the natural killermediated lysis of freshly isolated myeloid or lymphoblastic leukemias: evidence for the involvement of the poliovirus receptor (cd155) and nectin-2 (CD112)
2066 - 2073.
functional characterization of dnam-1 (cd226) interaction with its ligands pvr (cd155) and nectin-2 (prr-2/CD112)
533 - 538.