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Human beta Nerve Growth Factor Recombinant

NGF

accession:  P01138
   Size A     5ug   $ 70
   Size B      20ug  $160
   Size C      1mg  $ 2700
Domain  :  NGF
Gene  :  NGF
Catalog no. :  RKP01138
Source:
Optimized DNA sequence encoding Human Nerve Growth Factor mature chain was expressed in CHO cells.

 
Molecular weight:

Native human Nerve Growth Factor is generated by the proteolytic removal of the signal peptide and propeptide the molecule has a calculated molecular mass of approximately 27 kDa.

Recombinant human NGF is as a non disulfide-linked homodimeric protein consisting of  two 118 amino acid residue subunits, and migrates as a 13 - 14 kDa protein under reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the determined by its ability to proliferate TF-1 cells and was found to be in the range of  0.5 ng/ml.

 
Protein Sequence:
        10         20         30         40         50         60 
MSMLFYTLIT AFLIGIQAEP HSESNVPAGH TIPQAHWTKL QHSLDTALRR ARSAPAAAIA 

        70         80         90        100        110        120 
ARVAGQTRNI TVDPRLFKKR RLRSPRVLFS TQPPREAADT QDLDFEVGGA APFNRTHRSK 

       130        140        150        160        170        180 
RSSSHPIFHR GEFSVCDSVS VWVGDKTTAT DIKGKEVMVL GEVNINNSVF KQYFFETKCR 

       190        200        210        220        230        240 
DPNPVDSGCR GIDSKHWNSY CTTTHTFVKA LTMDGKQAAW RFIRIDTACV CVLSRKAVRR 


A 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
NGF-beta was lyophilized from a 0.2 μm filtered solution in 20mM sodium acetate, 150mM NaCl, pH 5.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Nerve Growth Factor

NGF is mainly responsible for the survival and the differentiation and the functional activities of sensory and sympathetic neurons in the peripheral nervous system. It also plays an important role in the development and functional activities of cholinergic neurons in the central nervous system. Since NGF is synthesized also in non-neuronal tissues it may have a much wider spectrum of biological activities than thought previously. NGF stimulates chemotactic migration of human polymorphonuclear leukocytes in vitro. NGF stimulates the growth and differentiation of B- cells and the growth of T- cells and of some tumor cell types. NGF inhibits immunoglobulin production by various human plasma cell. The cytokines IL1, IL6, and bFGF are potent inducers of NGF. NGF induces the synthesis of IL1 in pheochromocytoma cells which in turn acts as a growth factor for glial cells and induces the synthesis of NGF following nerve injuries. In thymic stromal cells NGF induces the synthesis of IL6. NGF induces the synthesis of the fos oncogene and the myc oncogene and also influences the expression of EGF. One receptor that is responsible for mediating most of the activities of NGF is expressed preferentially in neuronal tissues. This glycoprotein of 140 kDa is the product of the trk gene. It possesses an intrinsic tyrosine- specific protein kinase in its intracellular domain.

Related Publications:
in vitro evidence of Nerve Growth Factor effects on human conjunctival epithelial cell differentiation and mucin gene expression
Invest. Ophthalmol. Vis. Sci., Oct 2009; 50: 4622 - 4630.
identification of critical residues within the conserved and specificity patches of Nerve Growth Factor leading to survival or differentiation
J. Biol. Chem., Sep 2009; 10.1074/jbc.M109.058420.
neuroactive conducting scaffolds: Nerve Growth Factor conjugation on active ester-functionalized polypyrrole
J R Soc Interface, Sep 2009; 6: 801 - 810.
Nerve Growth Factor enhances cough and airway obstruction via trka receptor- and trpv1-dependent mechanisms
Thorax, Sep 2009; 64: 791 - 797.
erk5 activity is required for Nerve Growth Factor-induced neurite outgrowth and stabilization of tyrosine hydroxylase in pc12 cells
J. Biol. Chem., Aug 2009; 284: 23564 - 23573.




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