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bovine Basic Fibroblast Growth Factor Recombinant

bFGF

accession:  P03969
   Size A     10ug   $ 70
   Size B      50ug  $160
   Size C      1mg  $ 1300
Domain  :  HBGF/IL1
Gene  :  FGF2
Catalog no. :  RKP03969
Source:
Optimized DNA sequence encoding Bovine Basic Fibroblast Growth Factor mature chain was expressed in Escherichia Coli.

 
Molecular weight:
Native bovine bFGF is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated mass of approximately 17kDa.

Recombinant bovine basic FGF is a disulfide-linked monomer protein, consisting of  147 amino acids and migrates as an approximately 17 kDa protein under reducing conditions.


 
Purity:
>98%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the  proliferation of NR6R-3T3 fibroblasts, was found to be less than 0.1 ng/ml,corresponding to  3x106 IU/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI 

        70         80         90        100        110        120 
KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY 

       130        140        150 
SSWYVALKRT GQYKLGPKTG PGQKAILFLP MSAKS 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant bovine bFGF was lyophilized from a 0.2 μm filtered PBS solution pH 7.5.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

FGF-b

bFGF is the prototype of a large family of proteins, designated FGF family. Its members display a remarkable affinity to heparin and are therefore called also HBGF (heparin binding growth factors). bFGF is related to another member of the FGF family, aFGF and shows a homology of 55 % which is limited, however, to a few functional domains. The structure of bFGF, revealed by X-ray crystallography, shows a similar folding structure as IL1. The sequences of bovine and human bFGF differ in only 2 amino acids. Human and ovine bFGF are identical. A homolog of human bFGF isolated from Xenopus laevis shows an overall sequence homology of 84 %.

 




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Antibodies:
Purified Polyclonal
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