Cart contains    items

Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Monoclonal Mouse Anti Human GM-CSF


accession:  P04141
   Size A       $
   Size B      500ug  $400
   Size C      1mg  $ 600
Clone  : 
Isotype  :  IgG1
Catalog no. :  RMA04141
  • Neutralization: Data not available.
  • Western blot: This monoclonal antibody has been tested in WB analysis of human GM-CSF . A suitable range of concentrations of this antibody for WB detection is 0.5-2 µg/ml.
  • IHC: Use at a concentration of 1-2 µg/ml.
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with recombinant E. coli-derived Human GM-CSF. 
Species reactivity:

The IgG fraction of the tissue culture supernatant was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered solution at a concentration of 1mg/ml in citrate-phosphate buffer pH7.0, with 0.02%Proclin 300 as a preservative.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.


This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.



GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for the full spectrum of biological activities. Non- glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non- glycosylated protein. This protein is secreted together with other factors by T- cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM- CSF.

Related Publications:
GM-CSF regulates intimal cell proliferation in nascent atherosclerotic lesions
J. Exp. Med., Sep 2009; 206: 2141 - 2149.
the transcription factors stat5a/b regulate GM-CSF-mediated granulopoiesis
Blood, Sep 2009; 10.1182/blood-2009-04-216390.
GM-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace)
Blood, Sep 2009; 10.1182/blood-2009-04-215020.
biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation
PNAS, Sep 2009; 106: 15825 - 15830.
stress response genes are suppressed in mouse preimplantation embryos by granulocyte-macrophage colony-stimulating factor (GM-CSF)
Hum. Reprod., Sep 2009; 10.1093/humrep/dep307.

by class:
Growth factors
CD markers
Viral Antigens
by species:
Purified Polyclonal
Related Products:
product_icon_7.jpg Monoclonal Mouse Anti Human GM-CSF
product_icon_8.jpg Human GM-CSF Recombinant
product_icon_8.jpg Mouse GM-CSF Recombinant
product_icon_8.jpg Mouse GM-CSF Recombinant (CHO cell)
product_icon_8.jpg Human GM-CSF Recombinant (CHO cell)
product_icon_7.jpg Rabbit anti GM-CSF Polyclonal
product_icon_8.jpg Human CD116 Recombinant (GM-CSFR)
Item name Size Quantity Price(USD) Remove
Cart Total $