Biotinylated HLA-E*01:03 VMAPRTLVL peptide complex Tetramer
Categories: HLA-A peptide complexesMHC peptide complexes$1,140.00 – $3,800.00
Description
Accession
P13747
Source
DNA sequence encoding the Gly25-Ile305(HLA-E*01:03),Ile21-Met119(B2M)and VMAPRTLVL peptide.This complex includes a C terminal C-His-Avi tag and was expressed in HEK cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Biotinylated HLA-E*01:03 Tetramer VMAPRTLVL peptide complex is a glycosylated protein. The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Biotinylated HLA-E*01:03 Tetramer VMAPRTLVL peptide complex was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Q03519
Interactor
Interactor
Methods
Human HLA-E*01:03 Tetramer Protein Tris-Bis PAGE
- Human HLA-E*01:03 Tetramer Protein PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human HLA-E*01:03 Tetramer Protein SEC-HPLC
- The purity of Biotinylated Human HLA-E*01:03 Tetramer Proteinwas greater than 95% as determined by SEC-HPLC.
Biotinylated Human HLA-E*01:03 Tetramer Protein Tris-Bis PAGE
- Biotinylated Human HLA-E*01:03 Tetramer Protein PAGE under reduced condition. The purity is greater than 95%.
