Biotinylated HLA-A*11:01 VVVGASGVGK peptide complex
Categories: HLA-A peptide complexesMHC peptide complexes$210.00 – $850.00
Description
Accession
AAV53343.1
Source
DNA sequence encoding the Gly25-Thr305(HLA-A*11:01), Ile21-Met119(B2M)and VVVGASGVGK peptide.This complex includes a C terminal C-His-Avi tag and was expressed in HEK cells.
Molecular weight
Recombinant HLA-A*11:01 VVVGASGVGK peptide complex is a glycosylated protein. The protein has a predicted MW of 50.30 kDa. Due to glycosylation, the protein migrates to 53-63 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant HLA-A*11:01 VVVGASGVGK peptide complex was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Biotinylated Human KRAS G12S (HLA-A*11:01) Protein SEC-HPLC
- The purity of Biotinylated Human KRAS G12S (HLA-A*11:01) Proteinwas greater than 95% as determined by SEC-HPLC.
Biotinylated Human KRAS G12S (HLA-A*11:01) Protein Tris-Bis PAGE
- Biotinylated Human KRAS G12S (HLA-A*11:01) Protein PAGE under reduced condition. The purity is greater than 95%.
