DNA sequence encoding immunodominant fragment of Varicella Zoster virus Glycoprotein E was expressed in Escherichia Coli.
RecombinantVaricella Zoster virusgEencodes the immunodominant sequences of the VZV Glycoprotein E. This molecule has a calculated mass of approximately 11kDa. Recombinant VZVGlycoprotein Econtains an N terminal GST purification tag and migrates as an approximately 37 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE.
VZV gE antigen is suitable for ELISA and Western blots, excellent antigen for detection of VZV with minimal specificity problems.
Lyophilized from 25mM Tris-Hcl pH 8.0, 1mM EDTA and 50% glycerol.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.