DNA sequence encoding immunodominant fragment of Toxoplasma SAG1(p30) protein was expressed in Escherichia Coli.
Recombinant TOXGO SAG1(p30) encodes the immunodominant sequences of the Toxoplasma Dense Granule protein. Recombinant SAG1 contains an N terminal GST purification tag and migrates as an approximately 43 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE.
Toxoplasma SAG1 antigen is suitable for ELISA and Western blots, excellent antigen for detection of Toxoplasma with minimal specificity problems.
Lyophilized from 50mM Tris-HCl, 60mM NaCl and 50% glycerol.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.