Staphylococcal Protein A (SPA) Recombinant
Category: Uncategorized$160.00 – $1,600.00
Description
Accession
P02976
Source
Optimized DNA sequence encoding Staphylococcus Aureus IgG Binding domain repeat was expressed in Escherichia Coli.
Molecular weight
Recombinant Protein A, is a monomer protein consisting of 300 amino acid residue subunits. The molecule has a calculated molecular mass of approximately 34 kDa.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
Activity was determined by a binding capacity assay to human IgG, and was found to be not less than 95%.
Applications
Elisa,WB,Affinity purification of monoclonals and polyclonals,Immunoprecipitation.
Presentation
Recombinant Protein A was lyophilized from a 0.2 μm filtered solution.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function