Optimized DNA sequence encoding Rat Insulin Growth Factor -1mature chain was expressed in Escherichia Coli.
Native Rat Insulin Growth Factor-1 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 8kDa. Recombinant rat IGF-1 is a monomeric protein consisting of 70 amino acid residue subunits. IGF-1 migrates as an approximately 8kDa protein under non-reducing and reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The ED50 was determined by a cell proliferation assay using FDC-P1 cells is ≤.0 ng/ml, corresponding to a specific activity of ≥1 x units/mg.
MGKISSLPTQ LFKCCFCDFL KVKMHTMSSS HLFYLALCLL TFTSSATAGP ETLCGAELVD ALQFVCGPRG FYFNKPTGYG SSIRRAPQTG IVDECCFRSC DLRRLEMYCA PLKPTKSA RS VRAQRHTDMP KTQKEVHLKN ASRGSAGNKN YRM
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant Rat IGF1was lyophilized from a.2 μm filtered PBS solution, pH.4.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Impaired IGF1 signaling in fibroblasts that have phenotypic features of diabetes, inflammation or hypercortisolemia.
- Fibroblasts with phenotypic characteristics of type 2 diabetes (Diab) and those of control fibroblasts treated chronically with either vehicle (Cont), TNFα (4 ng/ml every day for 4 days) or dexamethasone (Dexa; 20 ng/ml every other day for 8 days) were serum starved for 24 hours prior to treatment for 15 minutes with IGF1 (50 ng/ml).