Optimized DNA sequence encoding rat GRO alpha mature chain was expressed in Escherichia Coli.
Native rat CXCL1/GRO-alpha is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa. Recombinant rat CXCL1/GRO-a is a monomer protein consisting of 72 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Determined by theability to chemoattract human neutrophils using a concentration range of.0-50.0 ng/ml.
MVSATRSLLC AALPVLATSR QATGAPVANE LRCQCLQTVA GIHFKNIQSL KVMPPGPHCT QTEVIATLKN GREACLDPEA PMVQKIVQKM LKGVPK
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant rat GRO-alpha/CXCL1was lyophilized from a.2μm filtered PBS pH.4.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.