Optimized DNA sequence encoding extracellular domain of Mouse TNF receptor type 2 (TNFR2) including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant Mouse TNF receptor 2 (CD120b) is a protein consisting of248 amino acid residue subunits,due to glycosylation migrates as an approximately 45kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Recombinant Mouse TNF receptor 2 (CD120b) activity was determined by the ability to inhibit the TNFα mediated cytotoxicity in the L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 1-3 μg/mL in the presence of 0.15 ng/mL recombinant mouse TNFα .
Recombinant Mouse TNFR2 (TNFRSF1B) is supplied as a 0.2 μm filtered PBS solution,pH7.2.
Recombinant Mouse TNF receptor 2 (CD120b), as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.