Description
Accession
Q80W65
Source
Optimized DNA sequence encoding extracellular domain of Mouse Proprotein convertase subtilisin/kexin type 9 (PCSK9) (GLN35 - GLN694) including an N-terminal His tag was expressed in HEK293 cells.
Molecular weight
Recombinant Mouse PCSK9 is a protein consisting of 670 amino acid residue subunits,due to glycosylation migrates as an approximately 20kDa & 60kDa protein on reduced SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity was tested by the ability of immobilized recombinant Mouse PCSK9 to bind recombinant human LDLR within a linear range of 10-100ng/ml in ELISA.
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Mouse PSCK9 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Storage
Recombinant PCSK9 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Biological Process
Molecular function
