Mouse Interleukin-21 Recombinant
Category: Recombinant Mouse Cytokines$160.00 – $4,700.00
Description
Accession
Q9ES17
Source
Optimized DNA sequence encoding Human Interleukin-21 mature chain was expressed in Escherichia Coli.
Molecular weight
Mouse Interleukin-21 is generated by the proteolytic removal of the signal peptide and propeptide, the molecule has a calculated molecular mass of approximately 15kDa. Recombinant Mouse IL-21 a monomeric protein consisting of 132 amino acid residue subunits, and migrates as an approximately 15kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>97%, as determined by SDS-PAGE and HPLC
Protein Sequence
MERTLVCLVV IFLGTVAHKS SPQGPDRLLI RLRHLIDIVE QLKIYENDLD PELLSAPQDV KGHCEHAAFA CFQKAKLKPS NPGNNKTFII DLVAQLRRRL PARRGGKKQK HIAKCPSCDS YEKRTPKEFL ERLKWLLQKM IHQHLS
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Interleukin-21 was lyophilized from a 0.2 μm filtered PBS solution pH7.5.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Molecular function
Methods
Exhausted CD8 T cells do not upregulate CD25 expression following IL-12, IL-18, and/or IL-21 exposure.
- Representative flow cytometry plots show the expression of CD25 by gated CD8 T cells following acute (B6 Arm), protracted (B6 cl13), and chronic (CD4-/- cl13) LCMV infections in response to a 5.5 hr incubation with or without IL-12, IL-18, and IL-21 in the absence of BFA.
Exhausted CD8 T cells do not upregulate CD25 expression following IL-12, IL-18, and/or IL-21 exposure.
- Representative flow cytometry plots show the expression of CD25 by gated CD8 T cells following acute (B6 Arm), protracted (B6 cl13), and chronic (CD4-/- cl13) LCMV infections in response to a 5.5 hr incubation with or without IL-12, IL-18, and IL-21 in the absence of BFA.
TGF-β enhances the effect of IL-21 on the development of IL-21-producing CD4+ T cells.
- Naïve CD4+ T cells from CBMCs were stimulated for 3 days with immobilized anti-CD3 and anti-CD28 mAbs (neutral condition) in the presence or absence of IL-21, TGF-β or IL-21 plus TGF-β.
The CD4+ T cell computational model predicts modulation of differentiation and maintenance by IL-21 in silico.
- Sensitivity analysis of IL-21 over Th1- and Th17-related molecules showing positive or negative correlation to IL-21.
The effect of IL-21 and CD40L exposure on MEC1 and MEC2 cells.
- Expression of EBNA-2 and LMP-1 in IL-21 treated cells .
