DNA sequence encoding the mature chain of Mouse IL31 including a C-terminal His tag was expressed in HEK293 Cells.
Recombinant Mouse IL31 is a protein consisting of amino acid residues [Thr 24-Cys163] ,due to glycosylation migrates as an approximately 23 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant IL31 was lyophilized from 0.2 µm filtered PBS solution pH7.4
TCSLSFGAPI SKEDLRTTID LLKQESQDLY NNYSIKQASG MSADESIQLP CFSLDREALT NISVIIAHLE KVKVLSENTV DTSWVIRWLT NISCFNPLNL NISVPGNTDE SYDCKVFVLT VLKQFSNCMA ELQAKDNTTC
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.