Optimized DNA sequence encoding extracellular domain of mouse IL-13 receptor alpha2 including an His tag was expressed in HEK293 cells.
Recombinant mouse IL-13 receptor alpha 2 is a monomer protein consisting of 325 amino acid residue subunits, due to glycosylation migrates as an approximately50kDa protein on SDS-PAGE.
>98%, as determined by SDS-PAGE and HPLC
The activity was measured by its ability to inhibit proliferation of IL-13 dependent TF1 Cells . The ED50 was determined to be 0.1-0.5 ug/ml.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant mouse IL-13 receptor alpha 2 is supplied as lyophilized 0.2 μm filtered PBS solution, pH7.2 .
Recombinant IL13RA2, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.