Optimized DNA sequence encoding extracellular domain of Mouse CD3ε including a flag tag was co-expressed with aDNA sequence encoding the extracellular domain of CD3δ including a C-terminal His tag, were expressed in HEK293 cells.
Recombinant mouse CD3 ε- CD3δ is a heterodimer protein consisting of 252 amino acid residue subunits, due to glycosylation migrates as an approximately 20kDa and as 26kDa bands on reduced SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse CD3ε-CD3δ is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant CD3 heterodimer can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.