Optimized DNA sequence encoding extracellular domain of Mouse CD200including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant Mouse CD200 is a monomer protein consisting of 213 amino acid residue subunits, due to glycosylation migrates as an approximately40-45kDa protein on SDS-PAGE.
>98%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant mouse CD200 (1ug/ml,100ul/well) to bind mouse CD200R1 with a linear range of 1-200 ng/ml
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD200is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
Recombinant CD200, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.