Optimized DNA sequence encoding mature chain of Mouse Angiopoietin-1 including an C-terminal His tag was expressed in HEK293 cells.
Recombinant Angpt1 is a protein consisting of 484 amino acid residue subunits, due to glycosylation migrates as an approximately 70kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse Angiopoietin-1 is lyophilized from 0.2 μm filtered 20mM SOdium Phosphate, pH7.5 ,200mM NaCl, 5% Trehalose.
A quick spin of the vial followed by gently mixing in distilled water to prepare a stock solution concentration not less than 0.1 mg/mL. Avoid vigorous shaking or vortex
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, the protein can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months, addition of a carrier protein to your working aliquots (0.1% BSA or HSA) is recommended for long term storage Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Schematic illustration of the key findings Flow restriction upon occlusion of a main arterial route results in downstream ischaemia and increases the shear forces on the vessel wall of upstream, preformed collateral vessels, inducing the release of different cytokines and chemokines (black triangles).
- Subsequently, ANG1 (black circles) induces transcriptional downregulation of Phd2, activating the NF-κB pathway.