Human Interleukin-18 Binding Protein (IL-18BP)Recombinant
Categories: CytokinesRecombinant Human Cytokines$160.00 – $1,950.00
Description
Accession
O95998
Source
Optimized DNA sequence encoding Human Interleukin-18 Binding Protein (IL-18BP) mature chain fused to an C-terminal polyHis tag was expressed in HEK cells.
Molecular weight
Native human IL-18BP, generated by the proteolytic removal of the signal peptide and propeptide,the molecule has a calculated molecular mass of approximately 19kDa. Recombinant Interleukin-18 Binding Protein is a monomer protein consisting of 163 amino acid residue subunits fused to a polyHis tag, migrates due to glycosylation as an approximately as 45kDa protein under non-reducing and reducing conditions in SDS-PAGE.
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
The activity of recombinant Interleukin-18 Binding Protein was determined by the ability to bind IL-18 in functional ELISA.
Protein Sequence
MTMRHNWTPD LSPLWVLLLC AHVVTLLVRA TPVSQTTTAA TASVRSTKDP CPSQPPVFPA AKQCPALEVT WPEVEVPLNG TLSLSCVACS RFPNFSILYW LGNGSFIEHL PGRLWEGSTS RERGSTGTQL CKALVLEQLT PALHSTNFSC VLVDPEQVVQ RHVVLAQLWA GLRATLPPTQ EALPSSHSSP QQQG
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant IL-18BP is supplied as an.2 μm filtered PBS solution.
Storage
Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
