Optimized DNA sequence encoding extracellular domain of human Insulin receptor including a C-terminal polyHis tag was expressed in HEK293 cells.
Recombinant human INSR is a monomer protein consisting of 928 amino acid residue subunits, due to glycosylation migrates as an approximately130kDa,45kDa protein under reducing SDS-PAGE conditions, corresponding to the alpha and beta receptor subunits.
>98%, as determined by SDS-PAGE and HPLC
Measured by its ability to bind recombinant human Insulin at a concentration range of 0.3-40ug/ml .
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant human Insulin receptor is supplied as lyophilized 0.2 μm filtered PBS solution, pH7.2 .
A quick spin of the vial followed by reconstitution in distilled water by gently mixing to a concentration not less than 0.1 mg/mL. Avoid harsh shaking or vortexing
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, the protein can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months, addition of a carrier protein to your working aliquots (0.1% BSA or HSA) is recommended for long term storage Avoid repeated freeze/thaw cycles. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.