Optimized DNA sequence encoding extracellular domain of human CD25 including a C-terminal polyHis tag was expressed in HEK293 cells.
Recombinant CD25 is a monomer protein consisting of 203 amino acid residue subunits, due to glycosylation migrates as an approximately44 kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
The activity of recombinant IL-2Ra (CD25) was determined by its ability to bind Human IL-2 with an affinity constant of 30nM .
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD25 is supplied as a 0.2 μm filtered PBS solution, pH7.4 .
A quick spin of the vial followed by reconstitution in distilled water to prepare a stock solution concentration not less than 0.1 mg/mL.
Recombinant CD25, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.