DNA sequence encoding Human IL27B fused toDNA sequence encoding mature chain of Human IL12 p35 via linker chain and Human IgG1 Fc C-terminal tag was expressed in CHO cells.
Recombinant Human Interleukin-35 is a monomer consisting of 645 amino acid residue , and migrates as an approximately95 kDa protein under reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE
The activity was measured using Immobilized Human IL-35 ability to bind biotinylated Human IL6RB in ELISA.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Interleukin-35 was lyophilized from a 0.2 μm filtered PBS solution pH 7.4.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.