Optimized DNA sequence encoding extracellular domain of human Interleukin-2 receptor beta subunit including an Fc tag was expressed in HEK293 cells.
Recombinant IL2RB is a monomer protein consisting of 451 amino acid residue subunits, due to glycosylation migrates as an approximately60kDa protein on SDS-PAGE.
Measured by its ability to bind biotinylated recombinant rat IL2 in a functional ELISA.
>97%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant IL-2RB is supplied as a lyophilized 0.2 μm filtered PBS solution, pH7.2 .
Recombinant IL2RB, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.