Optimized DNA sequence encoding Human GRO-gamma (CXCL3) mature chain was expressed in Escherichia Coli
Native human GRO-gamma is generated by the proteolytic removal of the signal peptide and propeptide. The molecule has a calculated molecular mass of approximately 8 kDa. Recombinant GRO-gamma is a disulfide-linked homodimeric protein consisting of 73 amino acid residue subunits, and migrates as an approximately 8 kDa protein under non-reducing and reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Determined by its ability to chemoattract transfected CXCR2 cells using a concentration range of-100 ng/ml.
MAHATLSAAP SNPRLLRVAL LLLLLVAASR RAAGASVVTE LRCQCLQTLQ GIHLKNIQSV NVRSPGPHCA QTEVIATLKN GKKACLNPAS PMVQKIIEKI LNKGSTN
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant GRO-gamma/CXCL3 was lyophilized from a.2μm filtered concentrated (1mg/ml) solution inmM NaCl,mM PB, pH7.0.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.