DNA sequence encoding the extracellular domain of Human EPOR including a C-terminal human IgG1-Fc tag was expressed in HEK293 Cells.
Recombinant Human EPOR is a protein consisting of amino acid residues [Ala 25-Pro250] ,due to glycosylation migrates as an approximately 37 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was determined by its ability to bind in functional ELISA assay.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant EPOR was lyophilized from 0.2 µm filtered PBS solution pH7.4
APPPNLPDPK FESKAALLAA RGPEELLCFT ERLEDLVCFW EEAASAGVGP GNYSFSYQLE DEPWKLCRLH QAPTARGAVR FWCSLPTADT SSFVPLELRV TAASGAPRYH RVIHINEVVL LDAPVGLVAR LADESGHVVL RWLPPPETPM TSHIRYEVDV SAGNGAGSVQ RVEILEGRTE CVLSNLRGRT RYTFAVRARM AEPSFGGFWS AWSEPVSLLT
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.