Optimized DNA sequence encoding Human extracellular domain of human CD16aincluding a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant CD16a is a monomer protein consisting of 203 amino acid residue subunits, due to glycosylation migrates as an approximately50 kDa protein on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
Recombinant CD16a activity was measured by the ability to bind human IgG with an affinity constant of 180nM.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant CD16a is supplied as a 0.2 μm filtered PBS solution, pH7.2 .
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL.
Recombinant CD16a, as supplied, can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to-70°Cfor twelve months. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.