DNA sequence encoding linear sequence 2-119AA of HCV core protein was expressed in Escherichia Coli.
Recombinant HCV Core encodes the immunodominant sequences of the HCV nucleocapsid p21 protein. This molecule has a calculated mass of approximately 13kDa.
>90%, as determined by SDS-PAGE and HPLC
Immunoreative with sera of infected HCV individuals.
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Sterile filtered from Tris-HCL 50mM pH 8.0, NaCl 60mM, Gluthatione 10mM, Sarcosyl 0.25%,glycerol 50%.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.