Optimized DNA sequence encoding Human BCMA extracellular domain fused to C terminal Human IgG1 Fc tag was expressed in CHO Cells.
Recombinant BCMA (CD269) Fc chimera is a homodimer protein consisting of amino acid residue subunits,and migrates as an approximately askDa band under non-reducing and askDa band under reducing conditions in SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The ED(50) was determined by the ability to bind immobilized recombinant Human BAFF and was calculated to beng/ml .
MLQMAGQCSQ NEYFDSLLHA CIPCQLRCSS NTPPLTCQRY CNASVTNSVK GTNA ILWTCL
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than.1 ng/µg(1EU/µg).
Recombinant BCMA was lyophilized from a.2 μm filteredmm Tris solutionpH.5.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than.1 mg/mL. This solution can then be diluted into other buffers.
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this product can be stored in working aliquots at° -° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.