Biotinylated Human B7-H6 Protein Recombinant
Category: Biotinylated proteins$542.00 – $1,805.00
Description
Accession
Q68D85
Source
DNA sequence encoding Human B7-H6 including a C-His-Avi tag was expressed in HEK293 Cells.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
The recombinant protein was then biotinylated site specific using the AVItag biotinylation technology.
Molecular weight
Recombinant Human B7-H6 is a protein consisting of amino acid residues [Asp25-Ser262] ,The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 48-65 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Human B7-H6 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Interactor
Methods
Biotinylated Human B7-H6 Protein Tris-Bis PAGE
- Biotinylated Human B7-H6 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Biotinylated Human B7-H6 Protein SEC-HPLC
- The purity of Biotinylated Human B7-H6 is greater than 95% as determined by SEC-HPLC.
Biotinylated Human B7-H6 Protein SPR Data
- Human NKp30, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human B7-H6, His-Avi Tag with an affinity constant of 0.227 μM as determined in SPR assay (Biacore T200).
