Optimized DNA sequence encoding I extracellular domain of Human Neuroligin-1 (NLGN1 ) (GLN46 - SER677 )including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Neuroligin-1 (NLG1) is a protein consisting of 640 amino acid residue subunits, due to glycosylation migrates as an approximately 110kDa protein on SDS-PAGE.
>90%, as determined by SDS-PAGE and HPLC
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human Neuroligin-1(NLGN1) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant Neuroligin-1 (NLG1) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Neuroligin-1 (NLG1) is a
molecule localized at
glutamatergic and GABAergic
synapses with a prominent
preference for the former .
At glutamatergic synapses,
NLG1 determines the number
of functional NMDAR and is
required for normal
expression of NMDAR-
dependent plasticity .
consists of a long
like domain that contains
two alternative splice
inserts, termed A and B,
followed by a short O-Glyc
Neuroligin-1 links neuronal activity to sleep-wake regulation
9974 - 9979.
Neuroligin-1 controls synaptic abundance of nmda-type glutamate receptors through extracellular coupling
725 - 730.
neurons get the Neuroligin-1 advantage
Neuroligin-1 induces neurite outgrowth through interaction with neurexin-1β and activation of fibroblast growth factor receptor-1
4174 - 4186.
neuroligin 1 is dynamically exchanged at postsynaptic sites
12733 - 12744.