Optimized DNA sequence encoding Human Kallikrein-6 - KLK6 (GLU17-LYS244 ) preprotein including a C-terminal His tag was expressed in HEK293 cells.
Recombinant Human Kallikrein-6 (hK6) is a protein consisting of 234 amino acid residue subunits,due to glycosylation migrates as an approximately as 32kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to cleave a fluorogenic substrate Boc-QARAMC .The specific activity was measured to be 250 pmole/ug/min
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Kallikrein-6 (hK6) is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Kallikrein-6 (KLK6) can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
The KLK6 gene is a new
of the human kallikrein gene
family and encodes for a
secreted protease, human
kallikrein 6 (hK6; also
Kallikrein 6, which has been
proposed as a novel
biomarker of ovarian cancer
, has been shown in vitro to
cleave substrates involved
in carcinogenesis .
Kallikrein 6 is also among
the members of the
kallikrein family with high
levels of expression in the
brain and related fluids
clinical significance and novel mechanism of action of kallikrein 6 in glioblastoma
305 - 318.
copy number and expression alterations of mirnas in the ovarian cancer cell line ovcar-3: impact on kallikrein 6 protein expression
296 - 305.
prognostic impact of cd68 and kallikrein 6 in human glioma
3269 - 3279.
differential n-glycosylation of kallikrein 6 derived from ovarian cancer cells or the central nervous system
Mol. Cell. Proteomics,
791 - 798.
kallikrein 6 induces e-cadherin shedding and promotes cell proliferation, migration, and invasion
8198 - 8206.