Optimized DNA sequence encoding mature chain of human alpha 1 antitrypsin -A1AT (GLU25-LYS418) including a C-terminal His tag was expressed in HEK293 cells.
Recombinant human alpha 1 antitrypsin (SERPIN A1) is a protein consisting of 385 amino acid residue subunits,due to glycosylation migrates as an approximately 55kDa band protein on reduced SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability to inhibit trypsin cleavage of a fluorogenic peptide substrate Mca-RPKPVE- Nval-WRKDnp-NH2, with a calculated ED50 < 4.5nM
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Human alpha 1 antitrypsin is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Recombinant Serpin A1 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
alpha 1 antitrypsin
AAT is a 52-kDa serine
synthesized primarily in the
liver and secreted into the
circulation with a half-life
of 45 days. AAT is the most
abundant circulating serine
The main substrate for AAT
is neutrophil elastase, a
protease secreted by
inflammation. AAT is
synthesized and secreted by
the liver and functions
primarily in the lungs,
where it protects pulmonary
tissue from degradation by
neutrophil elastase. A
deficiency of AAT results in
promiscuous activity of
resulting in early onset
emphysema and chronic
increased erk signalling promotes inflammatory signalling in primary airway epithelial cells expressing z α1-antitrypsin
Hum. Mol. Genet.,
performance of the bode index in patients with α1-antitrypsin deficiency-related copd
Eur. Respir. J.,
the circulating proteinase inhibitor α-1 antitrypsin regulates neutrophil degranulation and autoimmunity
Science Translational Medicine,
s64 circulating polymers are found in alpha-1-antitrypsin deficiency and are associated with lung disease
s63 f alpha-1 antitrypsin polymerises and promotes an exaggerated inflammatory response