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Mouse Angiotensinogen Recombinant

AGT

accession:  P11859
   Size A     20ug   $ 160
   Size B      100ug  $480
   Size C      500ug  $ 1950
Domain  : 
Gene  :  AGT
Catalog no. :  RKP11859

 

Source:

Optimized DNA sequence encoding mature chain of Mouse Angiotensinogen -AGT (ASP25 - VAL477) including an N-terminal His tag was expressed in HEK293 cells.


 
Molecular weight:

Recombinant mouse AGT is a protein consisting of 460 amino acid residue subunits,due to glycosylation migrates as an approximately 60kDa band protein on reduced SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant mouse Angiotensinogen (AGT) is lyophilized  from 0.2 μm filtered PBS solution,  pH7.2 ,  5% Trehalose.

              
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
Recombinant Angiotensinogen can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Angiotensinogen

Angiotensinogen is synthesized primarily in cells of the proximal tubule and is secreted from the apical surface into the lumen, where it is converted first to angiotensin (Ang) I and then Ang II by tubular renin and angiotensin- converting enzyme (ACE), respectively.

Related Publications:
association between urinary Angiotensinogen, hypertension and proteinuria in pregnant women with preeclampsia
Journal of Renin-Angiotensin-Aldosterone System, Feb 2014; 10.1177/1470320313510585.
dynamic ccaat/enhancer binding protein–associated changes of dna methylation in the Angiotensinogen gene
Hypertension, Feb 2014; 63: 281 - 288.
rock/nfb axis-dependent augmentation of Angiotensinogen by angiotensin ii in primary-cultured preglomerular vascular smooth muscle cells
Am J Physiol Renal Physiol, Jan 2014; 10.1152/ajprenal.00464.2013.
human Angiotensinogen +11525 c/a polymorphism modulates its gene expression through microrna binding
Physiol Genomics, Oct 2013; 45: 901 - 906.
epigenetic control of Angiotensinogen and cyp11b2 gene expression in the hypertrophic hearts
Eur. Heart J., Aug 2013; 34: P5748.




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product_icon_8.jpg Mouse Angiotensinogen Recombinant
product_icon_8.jpg Human Angiotensinogen Recombinant
 
 
 
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