Optimized DNA sequence encoding extracellular domain of Mouse CD27 including a C-terminal His tag was expressed in HEK293 cells.
Recombinant CD27 is a protein consisting of 175 amino acid residue subunits, due to glycosylation migrates as an approximately 20kDa protein on SDS-PAGE.
>97%, as determined by SDS-PAGE and HPLC
The activity was tested by the ability of immobilized recombinant Mouse CD27 to bind Human CD70 with a linear range of 0.08-1 ng/ml
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant Mouse CD27 is lyophilized from 0.2 μm filtered PBS solution, pH7.2 , 5% Trehalose.
Recombinant CD27 can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months.
Avoid repeated freeze/thaw cycles.
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
CD27 is a 120kDa type I
of disulfide-linked 55kDa
monomers and belongs to the
TNF receptor family.
CD27 is expressed on
medullary thymocytes (1,
20), on the surface of 75%
of human peripheral blood T
cells (equally distributed
between CD4+ and CD8+
subsets) (1, 2), on a
subpopulation of B cells,
and on NK cells. The
activation of human T cells
via the TCR-CD3 complex
leads to expression of CD27,
which peaks after 3 to 4
days and then gradually
decreases after prolonged
cd70 is downregulated by interaction with CD27 J. Immunol.,
2282 - 2289.
determination of the attp and attb sites of phage CD27 from clostridium difficile nctc 12727
J. Med. Microbiol.,
1439 - 1443.
cd8+ t cells produce the chemokine cxcl10 in response to CD27/cd70 costimulation to promote generation of the cd8+ effector t cell pool
the human fetal lymphocyte lineage: identification by CD27 and lin28b expression in b-cell progenitors
J. Leukoc. Biol.,
osteoclast precursors in murine bone marrow express CD27 and are impeded in osteoclast development by cd70 on activated immune cells
12385 - 12390.