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Rabbit anti IL-12 Polyclonal


accession:  P29460
   Size A     100ug   $ 290
   Size B      200ug  $390
   Size C      500ug  $ 590
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA29460
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
Polyclonal Interleukin-12 antibody was produced from sera of rabbits  immunized with synthetic protein containing Interleukin-12 C terminal antigenic determinants. 
Species reactivity:

Serum IgG fraction was purified by Protein G affinity chromatography.

Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.


This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.


IL12 is secreted by peripheral lymphocytes after induction. It is produced mainly by B-cells and to a lesser extent by T-cells. The most powerful inducers of IL12 are bacteria, bacterial products, and parasites. IL12 is produced after stimulation with Phorbol esters or Calcium ionophore by human B- lymphoblastoid cells. The IL12 receptor ,CD212, appears to be a single protein of approximately 110 kDa. Up to 1000-9000 high affinity IL12 receptors/cell are expressed on peripheral blood mononuclear cells activated by various mitogens for T-cells or by IL2. IL12 receptors are present on activated T-cells expressing CD4 and CD8 and on activated CD56(+) natural killer cells. Resting peripheral blood mononuclear cells, tonsillar B-cells, or tonsillar B-cells activated by anti-IgM/Dx, anti-IgM/Dx + IL2, or SAC + IL2 do not express the receptor. Human IL12 is not active in murine lymphocytes. Hybrid heterodimers consisting of murine p35 and human p40 subunits retain bioactivity on murine cells; however, the combination of human p35 and murine p40 is completely inactive on murine cells. Murine IL12 is active on both murine and human lymphocytes.

Related Publications:
gc1q receptor ligation selectively down-regulates Human IL-12 production through activation of the phosphoinositide 3-kinase pathway
J. Immunol., Oct 2005; 175: 4706 - 4714.
Human IL-12(p35) gene activation involves selective remodeling of a single nucleosome within a region of the promoter containing critical sp1-binding sites
Blood, Jun 2003; 101: 4894 - 4902.
impaired accumulation and function of memory cd4 t cells in Human IL-12 receptor 1 deficiency
J. Immunol., Jan 2003; 170: 597 - 603.
biosynthesis and posttranslational regulation of Human IL-12
J. Immunol., May 2000; 164: 4752 - 4761.
disparate intracellular processing of Human IL-12 preprotein subunits: atypical processing of the p35 signal peptide
J. Immunol., Jan 2000; 164: 839 - 847.

by class:
Growth factors
CD markers
Viral Antigens
by species:
Purified Polyclonal
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