Cart contains    items
SEARCH

  |
  |  
  |  
  |  
  |  
  |  
 
Browse by molecule: [A]  [B]  [C]  [D]  [E]  [F]  [G]  [H]  [I]  [J]  [K]  [L]  [M]  [N]  [O]  [P]  [Q]  [R]  [S]  [T]  [U]  [V]  [W]  [X]  [Y]  [Z]  

Rabbit anti IL-12 Polyclonal

IL-12

accession:  P29460
   Size A     100ug   $ 290
   Size B      200ug  $390
   Size C      500ug  $ 590
Clone  : 
Isotype  :  IgG
Catalog no. :  RPA29460
Applications:
Elisa: Use at a concentration range of  1-2ug/ml.
il-2 elisa Western blot:A suitable range of concentrations of this antibody for WB detection is 2-10 µg/ml.
il-2 elisa IHC: A suitable range of concentrations of this antibody for IHC  is 2-10 µg/ml.
 
Source:
Polyclonal Interleukin-12 antibody was produced from sera of rabbits  immunized with synthetic protein containing Interleukin-12 C terminal antigenic determinants. 
 
Species reactivity:
Mouse,Rat
 
Purification:

Serum IgG fraction was purified by Protein G affinity chromatography.

 
Presentation:
Lyophilized from sterile filtered PBS solution at a concentration of 1mg/ml.
 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water. This solution can then be diluted into other buffers
 
Storage:
The lyophilized antibody is stable for at least 1 year  from date of receipt at -20° C.
Upon reconstitution, this antibody can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:

This antibody product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

IL-12

IL12 is secreted by peripheral lymphocytes after induction. It is produced mainly by B-cells and to a lesser extent by T-cells. The most powerful inducers of IL12 are bacteria, bacterial products, and parasites. IL12 is produced after stimulation with Phorbol esters or Calcium ionophore by human B- lymphoblastoid cells. The IL12 receptor ,CD212, appears to be a single protein of approximately 110 kDa. Up to 1000-9000 high affinity IL12 receptors/cell are expressed on peripheral blood mononuclear cells activated by various mitogens for T-cells or by IL2. IL12 receptors are present on activated T-cells expressing CD4 and CD8 and on activated CD56(+) natural killer cells. Resting peripheral blood mononuclear cells, tonsillar B-cells, or tonsillar B-cells activated by anti-IgM/Dx, anti-IgM/Dx + IL2, or SAC + IL2 do not express the receptor. Human IL12 is not active in murine lymphocytes. Hybrid heterodimers consisting of murine p35 and human p40 subunits retain bioactivity on murine cells; however, the combination of human p35 and murine p40 is completely inactive on murine cells. Murine IL12 is active on both murine and human lymphocytes.

Related Publications:
gc1q receptor ligation selectively down-regulates Human IL-12 production through activation of the phosphoinositide 3-kinase pathway
J. Immunol., Oct 2005; 175: 4706 - 4714.
Human IL-12(p35) gene activation involves selective remodeling of a single nucleosome within a region of the promoter containing critical sp1-binding sites
Blood, Jun 2003; 101: 4894 - 4902.
impaired accumulation and function of memory cd4 t cells in Human IL-12 receptor 1 deficiency
J. Immunol., Jan 2003; 170: 597 - 603.
biosynthesis and posttranslational regulation of Human IL-12
J. Immunol., May 2000; 164: 4752 - 4761.
disparate intracellular processing of Human IL-12 preprotein subunits: atypical processing of the p35 signal peptide
J. Immunol., Jan 2000; 164: 839 - 847.




by class:
Cytokines
Chemokines
Growth factors
CD markers
Viral Antigens
Other
by species:
Human
mouse
Rat
Antibodies:
Purified Polyclonal
Related Products:
product_icon_8.jpg Human Interleukin-12 Recombinant
product_icon_7.jpg Rabbit anti IL-12 Polyclonal
product_icon_8.jpg Mouse IL-12 Recombinant
product_icon_8.jpg Human IL-12 receptor beta 1 Recombinant
product_icon_8.jpg Human IL-12B p40 Recombinant
 
 
 
Item name Size Quantity Price(USD) Remove
checkout
Cart Total $