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Human Oncostatin M Recombinant

OSM

accession:  P13725
   Size A     2ug   $ 70
   Size B      10ug  $160
   Size C      1mg  $ 4700
Domain  :  four helix cytokine core
Gene  :  OSM
Catalog no. :  RKP13725
Source:
Optimized DNA sequence encoding Human Oncostatin-M mature chain was expressed in Escherichia Coli.

 
Molecular weight:

Native human Oncostatin-M is generated by the proteolytic removal of the signal peptide and propeptide, this molecule has a calculated molecular mass of approximately 26 kDa.

Recombinant Oncostatin-M is a disulfide-linked homodimeric protein consisting of  228 amino acid residue subunits, and migrates as an approximately 26 kDa protein under  reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
The ED(50) was determined by the dose-dependent stimulation of the proliferation of f human TF-1 cells is ≤ 3 ng/ml, corresponding to a specific activity of ≥ 2 x 105 units/mg.

 
Protein Sequence:
        10         20         30         40         50         60 
MGVLLTQRTL LSLVLALLFP SMASMAAIGS CSKEYRVLLG QLQKQTDLMQ DTSRLLDPYI 

        70         80         90        100        110        120 
RIQGLDVPKL REHCRERPGA FPSEETLRGL GRRGFLQTLN ATLGCVLHRL ADLEQRLPKA 

       130        140        150        160        170        180 
QDLERSGLNI EDLEKLQMAR PNILGLRNNI YCMAQLLDNS DTAEPTKAGR GASQPPTPTP 

       190        200        210        220        230        240 
ASDAFQRKLE GCRFLHGYHR FMHSVGRVFS KWGESPNRSR RHSPHQALRK GVRRTRPSRK

       250 
GKRLMTRGQL  PR 
(*)Complete precursor sequence shown, expressed chain highlighted
 
Endotoxin:
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).

 
Presentation:
Recombinant Oncostatin M was lyophilized from a 0.2μm filtered concentrated (1mg/ml) PBS solution, pH 7.0.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

Oncostatin M

Oncostatin M is produced by monocytes and T-cells after cell activation, adherent macrophages, and various T- cell lines. Oncostatin M is a member of the IL6-related cytokine subfamily that includes IL6, IL11, LIF, CNTF and CT-1.Oncostatin M mediates its bioactivities through two different heterodimer receptors. They both involve gp130 as a signal transducing moiety, which is found also in receptors for a number of other cytokines. When gp130 dimerizes with LIF receptor beta subunit this generates the high affinity type 1 OSM receptor. When gp130 dimerizes with a protein known as OSM receptor beta this generates the type 2 OSM receptor. Both OSM receptors activate the receptor-associated Janus kinases JAK1, JAK2, and TYK2. Signaling also involves the transcriptional activators STAT3 and STAT5b (see also: STAT proteins. was specifically activated through the gp130-OSM-R-beta type II heterocomplex. The signaling pathway differences observed between the common type I LIF/OSM receptor and the specific type II OSM receptor might explain some of the bioactivities specifically displayed by OSM.Oncostatin M inhibits the growth of several tumor cell lines (A375 melanoma, lung carcinomas). The antiproliferative activity of oncostatin M for some cell lines is synergised by TGF-beta and IFN-gamma. It promotes the growth of human fibroblasts, vascular smooth muscle cells, and some normal cell lines. Oncostatin can inhibit the proliferation of murine M1 myeloid leukemic cells and induces their differentiation into macrophage-like cells, a function shared by LIF, G- CSF, and IL6.

Related Publications:
thrombin induces the expression of Oncostatin M via ap-1 activation in human macrophages: a link between coagulation and inflammation
Blood, Sep 2009; 114: 2812 - 2818.
Oncostatin M regulates secretoglobin 3a1 and 3a2 expression in a bidirectional manner
Am. J. Respir. Cell Mol. Biol., May 2009; 40: 620 - 630.
Oncostatin M enhances the antiviral effects of type i interferon and activates immunostimulatory functions in liver epithelial cells
J. Virol., Apr 2009; 83: 3298 - 3311.
Oncostatin M pathway plays a major role in the renal acute phase response
Am J Physiol Renal Physiol, Apr 2009; 296: F875 - F883.
unique methylation pattern of Oncostatin M receptor gene in cancers of colorectum and other digestive organs
Clin. Cancer Res., Mar 2009; 15: 1519 - 1526.




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