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Staphylococcal Protein A (SPA) Recombinant

SPA

accession:  P02976
   Size A     20mg   $ 160
   Size B      100mg  $500
   Size C      1gr  $ 1600
Domain  :  igG Binding
Gene  :  SPA
Catalog no. :  RKP02976
Source:
Optimized DNA sequence encoding Staphylococcus Aureus IgG Binding domain repeat  was expressed in Escherichia Coli.

 
Molecular weight:
Recombinant Protein A, is a monomer protein consisting of 300 amino acid residue subunits. The molecule has a calculated molecular mass of approximately 34 kDa. 

 
Purity:
>95%, as determined by SDS-PAGE and HPLC

 
Biological Activity:
Activity was determined by a binding capacity assay to human IgG, and was found to be not less than 95%.

              
 
Applications:

Elisa,WB,Affinity purification of monoclonals and polyclonals,Immunoprecipitation.

 

Presentation:
Recombinant Protein A was lyophilized from a 0.2 μm filtered solution.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this cytokine can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

protein a

Protein A is a 40-60 kDa MSCRAMM surface protein originally found in the cell wall of the bacteria Staphylococcus aureus.It binds with high affinity to human IgG1 and IgG2 as well as mouse IgG2a and IgG2b. Protein A binds with moderate affinity to human IgM, IgA and IgE as well as to mouse IgG3 and IgG1. It does not react with human IgG3 or IgD, nor will it react to mouse IgM, IgA or IgE.

Related Publications:
mcp-1 (monocyte chemotactic protein-1)-induced protein, a recently identified zinc finger protein, induces adipogenesis in 3t3-l1 pre-adipocytes without peroxisome proliferator-activated receptor
J. Biol. Chem., Oct 2009; 284: 27620 - 27628.
respiratory syncytial virus infection alters surfactant protein a expression in human pulmonary epithelial cells by reducing translation efficiency
Am J Physiol Lung Cell Mol Physiol, Oct 2009; 297: L559 - L567.
structure of the plasmodium falciparum circumsporozoite protein, a leading malaria vaccine candidate
J. Biol. Chem., Sep 2009; 284: 26951 - 26963.
bladder cancer associated protein: a potential prognostic biomarker in human bladder cancer
Mol. Cell. Proteomics, Sep 2009; 10.1074/mcp.M900294-MCP200.
maternal antibodies to pneumolysin, but not pneumococcal surface protein a, delay early pneumococcal carriage in high-risk papua new guinean infants
Clin. Vaccine Immunol., Sep 2009; 10.1128/CVI.00247-09.




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by species:
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Antibodies:
Purified Polyclonal
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