Optimized DNA sequence encoding extracellular domain of human IGF-1R (CD221) including a C-terminal 6His tag was expressed in HEK293 cells.
Recombinant human IGF-1R (CD221) is a heterodimer protein, due to glycosylation migrates as an approximately 50kDa,120kDa,150kDa protein under reducing ocnditions on SDS-PAGE.
>95%, as determined by SDS-PAGE and HPLC
The activity of recombinant IGF-1R was measured by its ability to bind Human IGF-1 in a functional ELISA assay.
Endotoxin content was assayed using a LAL gel clot method.
Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Recombinant IGF-1 receptor is supplied as a 0.2 μm filtered PBS solution, pH7.2.
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, the protein can be stored in working aliquots at 2° - 8° C for one month, or at -20°C to -70°C for twelve months, addition of a carrier protein to your working aliquots (0.1% BSA or HSA) is recommended for long term storage
Avoid repeated freeze/thaw cycles.
A quick spin of the vial followed by gently mixing in distilled water to prepare a stock solution concentration not less than 0.1 mg/mL.
Avoid vigorous shaking or vortex
Insulin-like growth factor 1
receptor (IGF-1R/CD221) is a
kinase receptor that plays
an important role in cell
growth, transformation, and
protection of cells from a
variety of apoptotic
stimuli. IGF-1R(CD221) is a
consisting of two -subunits
and two transmembrane β-
subunits that are linked by
disulfide bonds.The -
subunits are extracellular
and bind IGF. Each
contains an intracellular
tyrosine kinase domain.
Binding of IGF-I or IGF-II
ligand to IGF-1R leads to
phosphorylation of three key
tyrosine residues in the
kinase domain, leading to
autocrine igf-1/IGF-1R signalling is responsible for constitutive pi3k/akt activation in acute myeloid leukemia: therapeutic value of neutralizing anti-IGF-1R antibody
mir-29 micrornas regulate IGF-1R expression and contribute mantle cell lymphoma growth and survival.
Blood (ASH Annual Meeting Abstracts),
IGF-1R contributes to stress-induced hepatocellular damage in experimental cholestasis
Am. J. Pathol.,
627 - 635.
expression levels of total IGF-1R and sensitivity of nsclc cells in vitro to an anti-IGF-1R antibody (r1507)
ASCO Meeting Abstracts,
effect of IGF-1R inhibitor bms-754807 on tumor glucose metabolism and proliferation in sal-igf xenografts by fdg and flt- pet imaging
ASCO Meeting Abstracts,