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HIV-1 pol Integrase Recombinant

HIV1 Integrase

accession: 
   Size A       $
   Size B      0.5mg  $800
   Size C      1mg  $ 1100
Domain  :  Integrase
Gene  :  pol
Catalog no. :  RKP033661
Source:
DNA sequence encoding immunodominant full length fragment of HIV-1 Integrase was expressed in Escherichia Coli.

 
Molecular weight:

Recombinant HIV1 Integrase encodes the immunodominant sequence of the HIV Integrase protein. 

Recombinant HIV-1 Integrase contains an N terminal GST purification tag and migrates as an approximately 46 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.


 
Purity:
>95%, as determined by SDS-PAGE.
 
Applications:

HIV-1 Integrase antigen is suitable for ELISA and Western blots, excellent antigen for detection of HIV with minimal specificity problems.

 

Specificity:
Immunoreactive with sera of HIV-infected individuals.
 
Presentation:
Lyophilized from 20mM PBS pH-7.8, 20mM NaCl 0.5M, 1mM DTT & 8M urea.

 
Reconstitution:
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.

 
Storage:
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Upon reconstitution, this protein can be stored in working aliquots at 2° - 8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity.

Avoid repeated freeze/thaw cycles.

 
Usage:
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.

 

 

HIV1 Integrase

Integrase is a 288-amino acid protein (32 kDa) encoded by the end of the pol gene. It is produced as part of the Gag-Pol polypeptide precursor, from which it is released by viral protease-mediated cleavage. It has three independent domains. In addition to its well- documented role in integration of the viral genome, the HIV-1 enzyme IN (integrase) is thought to be involved in the preceding step of importing the viral cDNA into the nucleus. The ability of HIV to transport its cDNA through an intact nuclear envelope allows HIV- 1 to infect non-dividing cells, which is thought to be crucial for the persistent nature of HIV/AIDS.

 




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