Sheep LIF Recombinant
Categories: Food grade growth factorsSheep growth factors$300.00 – $1,900.00
Description
Accession
W5P8L4
Source
DNA sequence encoding Sheep LIF sp|W5P8L4|23-202AA was expressed in Ecoli.
Molecular weight
Recombinant Sheep LIF is a protein that migrates as an approximately 20kDa under reducing conditions in SDS-PAGE.
Purity
>96%, as determined by SDS-PAGE and HPLC
Biological Activity
The ED(50) was determined by the proliferation of Human TF1 cells was found to be < 1ng/ml .
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Sheep LIF was lyophilized from a 0.2 μm filtered solution in PBS, pH.
Reconstitution
A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
Storage
The lyophilized protein is stable for at least years from date of receipt at -20° C. Upon reconstitution, this cytokine can be stored in working aliquots at2° -8° C for one month, or at -20° C for six months, with a carrier protein without detectable loss of activity. Avoid repeated freeze/thaw cycles.
Usage
This cytokine product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
