Rhesus macaque LILRB1/CD85j/ILT2 Protein Recombinant
Category: Receptors$202.00 – $675.00
Description
Accession
NP_001035762
Source
DNA sequence encoding Rhesus macaque LILRB1/CD85j/ILT2 including a C-hFc tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Rhesus macaque LILRB1/CD85j/ILT2 is a protein consisting of amino acid residues [Met1-His474] ,The protein has a predicted MW of 75.2 kDa. Due to glycosylation, the protein migrates to 80-90 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Rhesus macaque LILRB1/CD85j/ILT2 was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Rhesus macaque LILRB1/CD85j/ILT2 Protein Tris-Bis PAGE
- Rhesus macaque LILRB1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Rhesus macaque LILRB1/CD85j/ILT2 Protein SEC-HPLC
- The purity of Rhesus macaque LILRB1 is greater than 95% as determined by SEC-HPLC.
Rhesus macaque LILRB1/CD85j/ILT2 Protein SPR Data
- Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G Complex Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200).
Rhesus macaque LILRB1/CD85j/ILT2 Protein SPR Data
- Anti-LILRB1 Antibody immobilized on CM5 Chip can bind Rhesus macaque LILRB1, hFc Tag with an affinity constant of 47.69 nM as determined in SPR assay (Biacore T200).
