Rhesus macaque Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein Recombinant
Category: Receptors$253.00 – $843.00
Description
Accession
F6QSK2
Source
DNA sequence encoding Rhesus macaque Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer including a C-His tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Rhesus macaque Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer is a protein consisting of amino acid residues [Phe31-Pro993(ITGAV)acidic tail & Glu43-Ser681(ITGB8)basic tail] ,The protein has a predicted MW of 112.1 kDa (ITGAV)&75.3 kDa (ITGB8). Due to glycosylation, the protein migrates to 90-140 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Rhesus macaque Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Rhesus macaque Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein Tris-Bis PAGE
- Rhesus macaque ITGAV&ITGB8 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Rhesus macaque Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein SEC-HPLC
- The purity of Rhesus macaque ITGAV&ITGB8 is greater than 95% as determined by SEC-HPLC.
Rhesus macaque Integrin alpha V beta 8 (ITGAV&ITGB8) Heterodimer Protein ELISA Data
- Immobilized Rhesus macaque ITGAV&ITGB8, His Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Anti-alpha-v Antibody , hFc Tag with the EC50 of 56ng/ml determined by ELISA.
