Rhesus macaque HLA-G Tetramer Protein Recombinant
Category: MHC peptide complexes$708.00 – $2,025.00
Description
Accession
O02948&RIIPRHLQL
Source
DNA sequence encoding Rhesus macaque HLA-G Tetramer including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Rhesus macaque HLA-G Tetramer is a protein consisting of amino acid residues [Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide] ,The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Rhesus macaque HLA-G Tetramer was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Rhesus macaque HLA-G Tetramer Protein Tris-Bis PAGE
- Rhesus macaque HLA-G Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.
Rhesus macaque HLA-G Tetramer Protein SEC-HPLC
- The purity of Rhesus macaque HLA-G Tetramer is greater than 95% as determined by SEC-HPLC.
Rhesus macaque HLA-G Tetramer Protein SPR Data
- Rhesus macaque LILRB1, hFc Tag captured on CM5 Chip via Protein A can bind Rhesus macaque HLA-G Tetramer, His Tag with an affinity constant of 1.74 nM as determined in SPR assay (Biacore T200).
