Rhesus macaque HLA-G Protein Recombinant
Category: MHC peptide complexes$253.00 – $843.00
Description
Accession
O02948&RIIPRHLQL
Source
DNA sequence encoding Rhesus macaque HLA-G including a C-His-Avi tag was expressed in HEK293 Cells.
Molecular weight
Recombinant Rhesus macaque HLA-G is a protein consisting of amino acid residues [Gly25-Thr305(HLA-G),Ile21-Met119(B2M)and RIIPRHLQL peptide] ,The protein has a predicted MW of 50.5 kDa. Due to glycosylation, the protein migrates to 58-63 kDa based on Tris-Bis PAGE result..
Purity
>95%, as determined by SDS-PAGE and HPLC
Biological Activity
see methods section
Endotoxin
Endotoxin content was assayed using a LAL gel clot method. Endotoxin level was found to be less than 0.1 ng/µg(1EU/µg).
Presentation
Recombinant Rhesus macaque HLA-G was lyophilized from 0.2 µm filtered PBS solution pH7.4
Reconstitution
Centrifuge vial to ensure maximal product extraction, recommended:20sec, 5K RPM
Storage
The lyophilized protein is stable for at least 2 years from date of receipt at -20° C.
Usage
This product is for research purposes only.It may not be used for therapeutics or diagnostic purposes.
Methods
Rhesus macaque HLA-G Protein Tris-Bis PAGE
- Rhesus macaque HLA-G on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.
Rhesus macaque HLA-G Protein SEC-HPLC
- The purity of Rhesus macaque HLA-G is greater than 95% as determined by SEC-HPLC.
